Oxidative modification of cellulosic fibres by lytic polysaccharide monooxygenase AA9A from Trichoderma reesei
نویسندگان
چکیده
Abstract Lytic polysaccharide monooxygenases (EC1.14.99.53-56, LPMOs) are oxidative enzymes with the capability to enhance lignocellulose saccharification as well nanofibrillation of cellulosic fibres. The parameters affecting efficiency modification cotton linters and softwood kraft fibres by LPMO from Trichoderma reesei ( Tr AA9A) were studied here. Circular dichroism experiments carried out analyze thermal stability indicate optimum temperature for experiments. enzyme was found be most thermostable at pH 5 but can tolerate up 40–50 °C neutral pH. enzymatic fibre oxidation dependent on reductant (gallic acid) hydrogen peroxide supply in a manner. A high resolution UHPLC-IM-MS method used identification oxidized oligosaccharides released treatments. microscopy applied analysis single dissolution kinetics AA9A pretreated fibres, demonstrating that treatment facilitated degraded surface structures, which prevent swelling. Graphical
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ژورنال
عنوان ژورنال: Cellulose
سال: 2022
ISSN: ['1572-882X', '0969-0239']
DOI: https://doi.org/10.1007/s10570-022-04648-w